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SP1 as Scaffold Platform
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Fulcrum SP takes advantage of the "self-assembly" characteristics of SP1 in order to produce novel compounds with improved functionality.
SP1 spontaneously forms an extremely stable 12-mer complex, maintaining it's self-assembly ability even when fused to other proteins. Fusion is achieved when the SP1 gene is fused with the target protein gene and expressed in E.coli, resulting in a display of multiple copies of the target protein on the surface of the SP nanostructure. Fusion with SP1 enhances stability, performance and applicability of the target protein. In addition, multiple copies of SP1 fusion proteins can also be used in a modular way to attach more than one specific target protein onto the SP scaffold, thus creating multi-functional protein entities.
Fulcrum SP offers its partners an opportunity to jointly develop SP Scaffold-based products in order to:
- Assist with protein stabilization and enabling target protein fused to SP1 to be purified using elevated temperature
- Enable multivalent display of functional protein domains. i.e. scAb, enzymes, growth factors and binding proteins - enhancing functionality by high avidity and stability.
- Develop single-step diagnostic reagents by combining antibodies or other binding protein molecules together with detecting enzymes such as HRP and AP
- Develop pharmaceutical applications for protein-drug targeting & stabilization and decrease of immunological response.
- Display multiple enzymes to facilitate step-wise reactions to allow close proximity between two enzymes that catalyze sequential reaction.
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